Cesar Lavoie

2025

Entemplate Switching Oligo

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March 3, 2025

Entemplate Switching Oligo – Our findings suggest that template switching plays an important role in the generation of spurious polyadenylation and support the need for more rigorous filtering of. The tso or (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse. This 5′ race protocol contains two steps. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2.

In the first step, cdna is synthesized using an rt primer that contains an adaptor of known. The complementarity between these consecutive rg bases and the 3' dc extension of the cdna molecule empowers the subsequent template switching [5]. In the first step, template switching reverse transcription reaction generates cdnas with a universal sequence of. However, if a large fraction of the.

Entemplate Switching Oligo

The cdna synthesis and amplification protocol contains two steps. Most currently used highly efficient single cell transcriptome sequencing approaches exploit the template switching activity of reverse transcriptases to add a. The simple version of a ts oligo is a dna oligo sequence that carries 3 riboguanosines (rgrgrg) at its 3' end [1].

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